Dealing with different methods for Kluyveromyces lactis β-galactosidase purification
نویسندگان
چکیده
منابع مشابه
Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger β-galactosidase
BACKGROUND The beta-galactosidase from Kluyveromyces lactis is a protein of outstanding biotechnological interest in the food industry and milk whey reutilization. However, due to its intracellular nature, its industrial production is limited by the high cost associated to extraction and downstream processing. The yeast-system is an attractive method for producing many heterologous proteins. Th...
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This paper investigates the production and optimization of β-galactosidase enzyme using synthetic medium by Kluyveromyces lactis NRRL Y-8279 in shake flask cultures. Among the different cell disintegration methods used, the highest specific activity was obtained when the cells were permeabilized using isoamyl alcohol. Response surface methodology was used to investigate the effects of four ferm...
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In view of a commonly known beneficial role and low stability of ascorbic acid, many efforts are constantly undertaken to produce its improved derivatives. This paper presents results on the synthesis of ascorbic acid galactoside using transgalactosylation properties of β-galactosidase from Kluyveromyces lactis and lactose as a donor of galactosyl moiety. The purpose of this study was to determ...
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The effect of glutaraldehyde on the conformational properties of Kluyveromyces lactis β-galactosidase was determined. The optimum substrate of the enzyme interchanged from lactose to sucrose when it reacted with a certain concentration of glutaraldehyde. Its mechanism was studied by detecting the changes in fluorescence absorption. This is the first discovery that β-galactosidase can be convert...
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Kluyveromyces lactis β-galactosidase (Kl-β-Gal) is one of the most important enzymes in the dairy industry. The poor stability of this enzyme limits its use in the synthesis of galactooligosaccharides (GOS) and other applications requiring high operational temperature. To obtain thermoresistant variants, a rational mutagenesis strategy by introducing disulphide bonds in the interface between th...
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ژورنال
عنوان ژورنال: Biological Procedures Online
سال: 1998
ISSN: 1480-9222
DOI: 10.1251/bpo4